Red/ET recombination and its biomedical applications / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 502-506, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-305241
ABSTRACT
Red/ET recombination, a powerful homologous recombination system based on the Red operon of lambda phage or RecE/ RecT from Rac phage, provides an innovative approach for DNA engineering. Deletion, insertion and mutation can be quickly and precisely performed on the target gene mediated by Red/ET recombination with PCR derived DNA fragments or oligonucleotides. This technical platform has extensive applications in biomedical field including bacterial artificial chromosome modification, gene knock-out construction and genetic modification of E. coli strains as well as some other kinds of microorganisms. Recently, Red/ET recombination was improved in several aspects so that it becomes more powerful and maneuverable. The characteristic and development of Red/ET recombination and its biomedical applications were described in this review.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Recombinases Rec A
/
Recombinação Genética
/
DNA Recombinante
/
Engenharia Genética
/
Bacteriófago lambda
/
Proteínas de Escherichia coli
/
Proteínas de Ligação a DNA
/
Escherichia coli
/
Exodesoxirribonucleases
/
Genética
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2005
Tipo de documento:
Artigo
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