Expression and detection of recombinant directing toxin SL120 in Pichia pastoris / 生物工程学报
Chinese Journal of Biotechnology
; (12): 473-477, 2005.
Article
em Zh
| WPRIM
| ID: wpr-305248
Biblioteca responsável:
WPRO
ABSTRACT
Anti-HIV-1 gp120 single chain antibody(scFv) gene and staphylococcus extoxin A(SEA) gene were inserted into vector pPIC9K. The recombinant plasmid was integrated into Pichia pastoris by electroporation. High level expression was performed by determining the Muts phenotype and screening muti-copy integrants. The recombinant protein was about 57kD and the production was 50.1 mg/L. It was shown that the two kinds of protein affected the conformation of each other by antibody affinity assay, but the recombinant targeting toxins could highly mediate CTLs to kill HIV-1 target cells.
Texto completo:
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Índice:
WPRIM
Assunto principal:
Pichia
/
Proteínas Recombinantes
/
Região Variável de Imunoglobulina
/
Proteína gp120 do Envelope de HIV
/
HIV-1
/
Enterotoxinas
/
Alergia e Imunologia
/
Vetores Genéticos
/
Genética
/
Metabolismo
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2005
Tipo de documento:
Article