Co-expression of gpd1 and hor2 from Saccharomyces cerevisiae in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 385-389, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-305264
ABSTRACT
Based on the principle of the pathway engineering, a novel pathway of producing glycerol was built in E. coli. The gpd1 gene encoding glycerol 3-phosphate dehydrogenase and the hor2 gene encoding glycerol 3-phosphatase were cloned from Saccharomyces cerevisiae, respectively. The two genes were inserted into expression vector pSE380 together. A recombinant plasmid pSE-gpd1-hor2 containing polycistron was constructed under the control of the strong trc promoter. Then it was transformed into E. coli BL21. The result showed the recombinant microorganism GxB-gh could convert glucose to glycerol directly. And the recombinant microorganism GxB-gh was incubated to produce glycerol from D-glucose in the fermentor. The maximal concentration of glycerol was 46.67g/L at 26h. Conversion rate of glucose was 42.87%. The study is about "green" producing glycerol by recombinant microorganism and is also useful for further working in recombining microorganism of producing 1,3-propanediol.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Saccharomyces cerevisiae
/
Proteínas Fúngicas
/
Engenharia Genética
/
Clonagem Molecular
/
Monoéster Fosfórico Hidrolases
/
Escherichia coli
/
Fermentação
/
Genética
/
Glicerol
/
Glicerolfosfato Desidrogenase
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2005
Tipo de documento:
Artigo
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