LC-ESI/MS identification and reduction of impurities in spiramycin fermentation / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 275-278, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-305286
ABSTRACT
There are several impurities in the spiramycin fermentation broth which leads to a lower yield and lower quality of the product. Four impurities in spiramycin broth have been simultaneously separated and identified by LC-ESI/MS. The generation of these impurities was attributed to the fluctuation of glucosylation in spiramycin biosynthesis. Nitrogen sources, ammonium in particular, were found to play an important role at the glucosylation. Aided with the information of LC-ESI/MS analysis and subsequent optimization of the culture medium, better culture medium of shake flask was designed, which leads to reduction of impurities by 22% - 88%.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Glicosilação
/
Espiramicina
/
Química
/
Meios de Cultura
/
Espectrometria de Massas por Ionização por Electrospray
/
Fermentação
/
Cromatografia Gasosa-Espectrometria de Massas
/
Métodos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2005
Tipo de documento:
Artigo
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