Expression and switching of TH1/TH2 type cytokines gene in human gliomas / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 268-272, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-305403
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression and switching of Th1/Th2 cytokines gene in human gliomas and its effects on occurring and developing of human gliomas.</p><p><b>METHODS</b>Interleukin (IL)-2 and interferon-gamma represent Th1 type cytokines. IL-4, IL-6, IL-10, and IL-13 represent Th2 type cytokines. The gene expressions of Thl/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes, and glioma cell lines were detected by reverse transcription polymerase chain reaction (RT-PCR). The biological activity of cytokines in the supernatant of glioma cell lines was assayed by enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>The total positive rates of Th1 and Th2 type cytokines gene in human glioma cells were 14.77% and 75%. The total positive rates of Th1 and Th2 type cytokines gene in glioma infiltrating lymphocytes were 22.73% and 68.17%. There was obviously predominant expression of Th2 type cytokines in human glioma tissues, glioma infiltrating lymphocytes, and glioma cell lines. There was no unbalanced expression of Th1/Th2 cytokines in normal brain tissues.</p><p><b>CONCLUSION</b>There is a predominant expression of Th2 type cytokines in human glioma cells. The switching of Thl/Th2 cytokines gene may play an important role in the occurring and developing of human gliomas.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Astrocitoma
/
Neoplasias Encefálicas
/
Regulação Neoplásica da Expressão Gênica
/
Citocinas
/
Interleucina-4
/
Interleucina-6
/
Interferon gama
/
Interleucina-2
/
Interleucina-10
Limite:
Adolescente
/
Adulto
/
Idoso
/
Criança
/
Criança, pré-escolar
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Chinese Medical Sciences Journal
Ano de publicação:
2005
Tipo de documento:
Artigo
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