Identification of differential genes in ovarian cancer using representational difference analysis of cDNA / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 185-189, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-305426
ABSTRACT
<p><b>OBJECTIVE</b>To identify differential genes between normal ovarian epithelium tissue and ovarian epithelial cancer using representational difference analysis of cDNA (cDNA-RDA).</p><p><b>METHODS</b>cDNA-RDA was performed to identify the differentially expressed sequences between cDNAs from cancer tissue and cDNAs from normal ovarian tissue in the same patient who was in the early stage of ovarian serous cystadenocarcinoma. These differentially expressed fragments were cloned and analyzed, then sequenced and compared with known genes.</p><p><b>RESULTS</b>Three differentially expressed cDNA fragments were isolated using cDNA from normal ovarian tissue as tester and cDNA from cancer tissue as driver amplicon by cDNA-RDA. DP III-1 and DP III-2 cDNA clone showed significant homology to the cDNA of alpha actin gene; DP III-3 cDNA clone showed significant homology to the cDNA of transgelin gene.</p><p><b>CONCLUSION</b>cDNA-RDA can be used to sensitively identify the differentially expressed genes in ovarian serous cystadenocarcinoma. Ovarian serous cystadenocarcinoma involves alteration of multiple genes.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Ovarianas
/
Ovário
/
DNA de Neoplasias
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Homologia de Sequência do Ácido Nucleico
/
Química
/
Genes Supressores de Tumor
/
Actinas
/
Análise de Sequência de DNA
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Sciences Journal
Ano de publicação:
2005
Tipo de documento:
Artigo
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