Regular expression of discoidin domain receptor 2 in the improved adjuvant-induced animal model for rheumatoid arthritis / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 133-137, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-305439
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of discoidin domain receptor 2 (DDR2) of fibroblast-like synovial cells in improved adjuvant-induced animal (AIA) model for rheumatoid arthritis (RA) and to provide evidence for DDR2's antagonist use clinically.</p><p><b>METHODS</b>AIA was modified by administrating 0.1 mL of complete Freund's adjuvant (CFA, mixed with 5 mg Bacillus Calmette-Guerin vaccine/mL) into rats' right hind paws and 0.125 mL tumor necrosis factor-alpha (2 U/mL) into right ankles and subpatellar fatty tissue. The expression of DDR2 in fibroblast-like synovial cells was assessed using immunohistochemistry, immunofluorescence histochemistry, and in situ hybridization methods. Levels of anti-collagen II antibody were measured using enzyme-linked immunosorbent assay.</p><p><b>RESULTS</b>Given the terms mentioned above, we found a more practical rat model, apparently decreasing immunization time (average 3-5 days). DDR2 can be detected upon the 15th day of immunization; expression gradually increased with time going on, and reaching a peak 35 days after immunization before gradually decreasing. Serum anti-collagen II antibody showed similar expression patterns as DDR2, but reached peak later than DDR2, about 40 days after immunization.</p><p><b>CONCLUSION</b>Regular expression of DDR2 in animal models infers its important role in the pathological process of RA.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Artrite Experimental
/
Artrite Reumatoide
/
Líquido Sinovial
/
Sangue
/
Vacina BCG
/
Receptores Mitogênicos
/
Adjuvante de Freund
/
Ratos Sprague-Dawley
/
Receptores Proteína Tirosina Quinases
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Sciences Journal
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS