Expression, purification, and characterization of an anti-human RBC ScFv-HIV gp160 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 76-78, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-305489
ABSTRACT
<p><b>OBJECTIVE</b>To construct and express anti-human RBC and HIVgp160 fusion protein for rapid detection of antibody to HIV.</p><p><b>METHODS</b>The gene of the anti human RBC ScFv and HIV antigen were constructed together into expression vector. The fusion protein was expressed in E. coli.</p><p><b>RESULTS</b>The fusion protein was proved to be able to bind both anti-RBC and HIVgp160. It could cause agglutination of human RBC when HIVgp160 was present.</p><p><b>CONCLUSION</b>The fusion protein has the potential in rapid detection of HIV.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Autoanticorpos
/
Sangue
/
Proteínas Recombinantes de Fusão
/
Ensaio de Imunoadsorção Enzimática
/
Testes de Hemaglutinação
/
Anticorpos Anti-HIV
/
Expressão Gênica
/
Soropositividade para HIV
/
Clonagem Molecular
/
Proteína gp160 do Envelope de HIV
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2007
Tipo de documento:
Artigo
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