Application of single radial hemolysis technique for diagnosis of influenza A (H5N1) / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 3-6, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-305540
ABSTRACT
<p><b>BACKGROUND</b>To understand the optimal condition of single radial hemolysis (SRH) for diagnosis of avian influenza A (H5N1) virus in order that SRH could be performed in general laboratories.</p><p><b>METHODS</b>The effect of different concentration of virus and species of red blood cells, as well as kind and concentration of agarose on testing sensitivity of SRH was determined. Meanwhile the sensitivity and specificity of this method were compared with those of micro-neutralization test.</p><p><b>RESULTS</b>The optimal condition of SRH included the viral concentration of 1000 HA units per 0.1 ml packed chicken red blood cells, the agarose concentration of 1.0%, the compliment added into agarose-virus-rbc slides after diffusion of sera. The sensitivity and specificity of SRH were very similar to those of micro-neutralization test. Meanwhile, no cross reaction between antibodies, especially antibodies against N1 antigens, H5N1 and H1N1 viruses was detected.</p><p><b>CONCLUSION</b>The sensitivity and specificity of SRH were very similar to those of micro-neutralization assay. SRH could be performed in normal laboratories and be used for testing large scale serum samples.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Testes de Inibição da Hemaglutinação
/
Testes de Neutralização
/
Infecções por Orthomyxoviridae
/
Diagnóstico
/
Alergia e Imunologia
/
Influenza Humana
/
Virus da Influenza A Subtipo H5N1
/
Cobaias
/
Anticorpos Antivirais
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2006
Tipo de documento:
Artigo
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