Isolation and identification of arboviruses in Hebei Province / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 52-55, 2006.
Article
em Zh
| WPRIM
| ID: wpr-305546
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>To study the arboviruses carried by mosquitoes collected in Hebei Province.</p><p><b>METHODS</b>Samples were collected from mosquito active sites and stored in liquid nitrogen till use. Pools of 20 to 30 mosquitoes were ground after sterilization, centrifugal supernant was inoculated onto C6/36 cell, cytopathic effect was observed for three sequential passages. Positive isolates were identified by IFA and RT-PCR.</p><p><b>RESULTS</b>Totally 1310 mosquitoes were collected from two villages of She county, Hebei province. They were divided into 46 pools and ground respectively. Thirteen positive isolates were obtained. Two isolates reacted with alphaviral antibodies and were amplified by alphaviral primers, nucleotide sequence showed the highest homology (98%) to Getah virus (AY702913.1), so the two isolates were identified as Getah virus.</p><p><b>CONCLUSION</b>Getah virus was isolated from mosquitoes in Hebei Province. This is the first report of isolating Getah virus from inland of China.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Filogenia
/
Arbovírus
/
Virologia
/
Análise por Conglomerados
/
Linhagem Celular
/
Classificação
/
Análise de Sequência de DNA
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Genética
/
Culicidae
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2006
Tipo de documento:
Article