Location of Dctn1 in the mouse testis and sperm and its role in spermiogenesis / 中华男科学杂志
National Journal of Andrology
; (12): 799-804, 2011.
Article
em Zh
| WPRIM
| ID: wpr-305787
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of dynactin 1 (Dctn1) in the process of mouse spermiogenesis.</p><p><b>METHODS</b>Western blot and indirect immunofluorescence were used to analyze the expression and location of Dctn1 in the mouse testis and spermatozoa. The highest efficiency of small interference RNA (siRNA) was verified by GC2-spd cell line in vitro and in vivo studies, respectively. Dctn1 siRNA mixed with the indicator (0.4% trypan blue) was injected into the seminiferous tubules of 3-week-old ICR mice through rete testis microinjection, and negative control siRNA injected into the control testes. The normal group included 3-week-old ICR mice that did not receive any treatment. Spermatozoa were collected from the cauda epididymis 3 weeks after siRNA injection for morphological analysis.</p><p><b>RESULTS</b>Dctn1 was mainly localized in the tail of spermatozoa. After interference, the sperm tail abnormality in the Dctn1 siRNA group was (23.57 +/- 0.55)%, significantly higher than (12.35 +/- 2.29)% in the control (P < 0.01, n = 3), and it was (3.37 +/- 0.69)% in the normal group.</p><p><b>CONCLUSION</b>Dctn1 plays an important role in mouse spermiogenesis, and mainly affects the formation of the tail of spermatozoa.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Rede do Testículo
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Túbulos Seminíferos
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Contagem de Espermatozoides
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Motilidade dos Espermatozoides
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Espermatogênese
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Espermatozoides
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Testículo
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RNA Interferente Pequeno
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Complexo Dinactina
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Genética
Limite:
Animals
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2011
Tipo de documento:
Article