Expression of annexin A7 in spermatogonial stem cells / 中华男科学杂志
National Journal of Andrology
;
(12): 516-522, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-305853
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of Annexin A7 in the mouse testis, especially in different types of spermatogonia.</p><p><b>METHODS</b>We prepared Annexin A7 recombinant protein using prokaryotic expression, adsorbed the Annexin A7 antibody with it after identified by mass spectrometry, and detected the expression of Annexin A7 by Western-blot and immunohistochemistry.</p><p><b>RESULTS</b>Annexin A7 was expressed in a development-dependent manner in the spermatogonia of the prepubertal mice and in the type-A single (As) and type-A paired (Apr) spermatogonia of adult mice. These results were confirmed by the co-localization of Annexin A7 and Stra8, a known determinant of differentiated spermatogonial stem cells (SSCs).</p><p><b>CONCLUSION</b>Annexin A7 is the internal factor of As and Apr spermatogonia, which might be involved in the biological functions of SSCs.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Espermatogônias
/
Células-Tronco
/
Anexina A7
/
Biologia Celular
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2011
Tipo de documento:
Artigo
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