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Adenovirus mediated antisense c-myc gene on the chemotherapy sensitivity of osteosarcoma cells to cisplatin / 中华外科杂志
Zhonghua Wai Ke Za Zhi ; (12): 799-802, 2005.
Article em Zh | WPRIM | ID: wpr-306206
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the recombinant adenovirus encoding antisense c-myc fragment and to investigate its effect on the chemotherapy sensitivity of osteosarcoma MG-63 cells to cisplatin.</p><p><b>METHODS</b>The recombinant adenovirus (Ad-Asc-myc) encoding antisense c-myc fragment was constructed by cloning c-myc cDNA of about 720 base pairs in a reverse direction into adenovirus vector, then undergoing recombination, amplifying and being complemented in vivo. The osteosarcoma MG-63 cells were transfected by the Ad-Asc-myc in vitro, and Wright staining, Acridine Orange staining, Western Blot, MTT, Flow Cytometry (FCM) were used to study cell morphology, expression of c-myc protein, tumor cell proliferation in vitro, apoptosis and cell cycle change.</p><p><b>RESULTS</b>Ad-Asc-myc encoding antisense c-myc fragment was obtained with the titer of 2 x 10(9) pfu/ml. Ad-Asc-myc down-regulated the expression of c-myc protein after transfected MG-63 cells for 48 h, combined with the treatment of 2.0, 5.0 microg/ml cisplatin for 2 h could inhibit tumor cells proliferation in vitro by 33.4% and 54.2% respectively, which were significantly difference compared with control recombinant adenovirus (Ad-LacZ) groups (P < 0.05). Acridine Orange staining and FCM analysis showed that Ad-Asc-myc could induce apoptosis of transfected cells, which was enhanced by the treatment of cisplatin cell. Cycle analysis showed that obvious G2/M phase arrested in transfected cells.</p><p><b>CONCLUSION</b>Ad-Asc-myc increases the chemotherapy sensitivity of osteosarcoma MG-63 cells to cisplatin as well as induced apoptosis.</p>
Assuntos
Texto completo: 1 Índice: WPRIM Assunto principal: Patologia / Farmacologia / Recombinação Genética / Células Tumorais Cultivadas / Transfecção / Osteossarcoma / Adenovírus Humanos / DNA Antissenso / Genes myc / Proteínas Proto-Oncogênicas c-myc Limite: Humans Idioma: Zh Revista: Zhonghua Wai Ke Za Zhi Ano de publicação: 2005 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Patologia / Farmacologia / Recombinação Genética / Células Tumorais Cultivadas / Transfecção / Osteossarcoma / Adenovírus Humanos / DNA Antissenso / Genes myc / Proteínas Proto-Oncogênicas c-myc Limite: Humans Idioma: Zh Revista: Zhonghua Wai Ke Za Zhi Ano de publicação: 2005 Tipo de documento: Article