Desmoplakin expression silencing affects cardiac voltage-gated sodium channel Nav1.5 in HL-1 cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 983-989, 2013.
Article
em Zh
| WPRIM
| ID: wpr-306425
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the association of desmoplakin with the distribution and function of Nav1.5 by RNA silencing technology in HL-1 cells.</p><p><b>METHODS</b>HL-1 cells with desmoplakin expression suppression by RNA silencing were examined for desmoplakin and Nav1.5 protein expressions by Western blotting, and the distribution and co-location of desmoplakin and Nav1.5 protein were detected by immunofluorescence staining. Patch-clamp recording was applied to analyze the changes in whole-cell sodium current after desmoplakin silencing.</p><p><b>RESULTS</b>Compared with the untreated group and negative control group, the cells with desmoplakin silencing showed obviously reduced expressions of desmoplakin and Nav1.5 proteins. Co-localization of desmoplakin and Nav1.5 was detected at cell-cell contact in untreated and control conditions, and desmoplakin expression silencing induced a drastic redistribution of Nav1.5 with decreased peak current density (156.3∓6.2 vs 41.8∓3.1, n=6, P<0.05), a shift in voltage dependence of steady-state inactivation (-42 mV vs -61 mV, n=5, P<0.05), and prolonged time of recovery from inactivation.</p><p><b>CONCLUSION</b>Desmoplakin silencing caused redistribution of Nav1.5 protein and also changes in its electrophysiological properties in HL-1 cells.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Linhagem Celular
/
Inativação Gênica
/
Miócitos Cardíacos
/
Desmoplaquinas
/
Genética
/
Metabolismo
/
Mutação
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2013
Tipo de documento:
Article