Effect of dexamethasone on expression of interleukin-21 and phospho-STAT3 in a murine model of chronic asthma / 南方医科大学学报
Journal of Southern Medical University
;
(12): 742-745, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-306476
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of dexamethasone on the expression of interleukin-21 (IL-21) and phospho- STAT3 (p-STAT3) in a murine model of chronic asthma.</p><p><b>METHODS</b>Thirty-three female BALB/C mice were randomly divided into control group, asthmatic group and dexamethasone-treated group (n=11). In the latter two groups, asthmatic models were established by ovalbumin administration. The cells in the broncho-alveolar lavage fluid (BALF) were counted and classified. The airway inflammation was evaluated by HE staining, and the expressions of IL-21 and p-STAT3 in the lung tissue were detected by immunohistochemistry and Western blotting.</p><p><b>RESULTS</b>The total cell number and lymphocyte number in the BALF were significantly higher in asthmatic group than in the control group and dexamethasone group (P/0.01). Compared with the control group, the asthmatic group showed significantly increased protein expressions of IL-21 and p-STAT3 (P<0.05), which were reduced by dexamethasone intervention (P/0.05).</p><p><b>CONCLUSION</b>Dexamethasone can inhibit the expression of IL-21 and p-STAT3 in the murine model of chronic asthma, suggesting the importance of IL-21/STAT3 signaling pathway in the therapeutic mechanisms of dexamethasone for asthma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fosforilação
/
Asma
/
Dexametasona
/
Transdução de Sinais
/
Interleucinas
/
Modelos Animais de Doenças
/
Fator de Transcrição STAT3
/
Pulmão
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2013
Tipo de documento:
Artigo
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