The identification of the Cryptosporidium ubiquitum in pre-weaned Ovines from Aba Tibetan and Qiang autonomous prefecture in China / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 315-320, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-306857
ABSTRACT
<p><b>OBJECTIVE</b>Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qiang Autonomous Prefecture in the Sichuan province of China.</p><p><b>METHODS</b>A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically (following modified acid fast staining). In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed.</p><p><b>RESULTS</b>The prevalence of Cryptosporidium in the collected samples was at 14.6% (31/213) and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype (Cryptosporidium ubiquitum) demonstrating that this species was the primary sheep species found in sheep in China.</p><p><b>CONCLUSION</b>The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Parasitologia
/
Desmame
/
Ovinos
/
China
/
Reação em Cadeia da Polimerase
/
Cryptosporidium
/
Oocistos
/
Fezes
/
Genética
/
Microbiologia
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2011
Tipo de documento:
Artigo
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