Effects of mercury on the structure and activity of BLM642-1290 recombinant helicase / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 47-55, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-306892
ABSTRACT
<p><b>OBJECTIVE</b>Bloom's syndrome is an autosomal recessive disorder characterized by genomic instability and a predisposition to many cancers. Mutations of the BLM gene (encoding a BLM helicase) may form a structure of the etiology of this disease. As a global pollutant, mercury poses a major threat to human health. The current study was conducted to elucidate the effects of Hg(2+) on the structure and activity of BLM642-1290 recombinant helicase, and to further explore the molecular mechanisms of mercury toxicity to the DNA helicase.</p><p><b>METHODS</b>The effects of Hg(2+) on biological activity and structure of BLM642-1290 recombinant helicase were determined by fluorescence polarized, ultraviolet spectroscopic, and free-phosphorus assay technologies, respectively.</p><p><b>RESULTS</b>The helicase activity, the DNA-binding activity, and the ATPase activity of BLM642-1290 recombinant helicase were inhibited by Hg(2+) treatment. The LMCT (ligand-to-metal charge transition) peaks of the helicase were enhanced with the increase of the Hg(2+) level. The LMCT peaks of the same concentration of helicase gradually increased over time.</p><p><b>CONCLUSION</b>The biological activity of BLM642-1290 recombinant helicase is inhibited by Hg(2+) treatment. The conformation of the helicase is significantly altered by Hg(2+). There exist two binding sites between Hg(2+) and the helicase, which are located in the amino acid residues 1063-1066 and 940-944 of the helicase, respectively.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Conformação Proteica
/
Espectrofotometria Ultravioleta
/
Relação Estrutura-Atividade
/
Proteínas Recombinantes
/
Sequência de Bases
/
Química
/
Adenosina Trifosfatases
/
Primers do DNA
/
Toxicidade
/
RecQ Helicases
Limite:
Humanos
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2011
Tipo de documento:
Artigo
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