Effects of peroxisome proliferators activated receptors on caveolin-1 expression in foam cells / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 661-665, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-307224
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of peroxisome proliferators activated receptors (PPAR) alpha, gamma ligand on ATP-binding cassette transporter A1 (ABCA1) and caveolin-1 expressions and cholesterol, ox-LDL contents in human monocyte derived foam cells.</p><p><b>METHOD</b>Malondialdehyde (MDA) was measured by TBARS method, ox-LDL detected by ELISA method, cholesterol measured by fluorescence spectrophotometric method, ABCA1, caveolin-1 mRNA and protein expressions determined by RT-PCR and Western blot, in human monocytes, foam cells [human monocyte-derived macrophage induced by myristate acetate (PMA) further treated with 50 mg/L ox-LDL for 24 h], foam cells plus 10 micromol/L pioglitazone for 48 h, foam cells plus 5 micromol/L clofibrate for 48 h.</p><p><b>RESULT</b>The intracellular total cholesterol (TC), free cholesterol (FC), cholesteryl ester (CE), ox-LDL and lipid peroxide were significantly increased and the membrane expressions of ABCA1, caveolin-1 were down-regulated in foam cells compared to monocytes (all P < 0.05) and these changes were significantly attenuated by cotreatment with PPARalpha, gamma ligand.</p><p><b>CONCLUSION</b>The anti-atherosclerosis effects of PPARalpha, gamma ligand are related to reducing cholesterol contents and up-regulating ABCA1, caveolin-1 expressions in foam cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Monócitos
/
Expressão Gênica
/
Linhagem Celular
/
Colesterol
/
Transportadores de Cassetes de Ligação de ATP
/
PPAR alfa
/
PPAR gama
/
Caveolina 1
/
Transportador 1 de Cassete de Ligação de ATP
/
Células Espumosas
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2007
Tipo de documento:
Artigo
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