Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2227-2231, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-307479
ABSTRACT
<p><b>OBJECTIVE</b>Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua.</p><p><b>METHOD</b>A P450 cDNA gene was cloned from A. annua by RT-PCR. The bioinformatics analysis of the P450 gene was performed.</p><p><b>RESULT</b>The complete ORF of this P450 cDNA is 1 464 bp and encodes 488 aa. The sequence was reported to GenBank and coded as DQ667171. Bioinformatics analysis of the P450 cDNA showed it encoded an A-type P450 protein with 54. 992 kDa, it's isoelectric point was 8.83 and the possibility of export to mitochondria was 0.893 2.</p><p><b>CONCLUSION</b>The comparable analysis of the P450 with CYP71AV1 revealed that the two proteins probably performed the same function because of the similar character.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Proteínas de Plantas
/
Plantas Medicinais
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Química
/
Fases de Leitura Aberta
/
Sequência de Aminoácidos
/
Classificação
/
Clonagem Molecular
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2007
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS