Effects of Salvia miltiorrhiza on TGF-beta1, ROS and PAI-1 induced by angiotensin II in renal mesangial cells / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of Salvia miltiorrhiza on glomerulosclerosis induced by Ang II.</p><p><b>METHOD</b>Rat mesangial cells were exposed to 100 nmol L(-1) Ang II. Meanwhile, we added S. miltiorrhiza injection of different concentrations to Mcs. PAI-1 mRNA and protein, TGF-beta1 in serum free MEM medium, and the level of cellular reactive oxygen species (ROS) were measured.</p><p><b>RESULT</b>S. miltiorrhiza notably attenuated Ang II induced expression of PAI-1 in a concentration-dependent manner. Meanwhile, S. miltiorrhiza suppressed the production of TGF-beta1 and cellular ROS in mesangial cells.</p><p><b>CONCLUSION</b>S. miltiorrhiza can alleviate glomerular sclerosis. The renoprotective effects of S. miltiorrhiza may be due to its ability to decrease Ang II -induced PAI-1 and TGF-beta1 secretion and cellular ROS level.</p>