Determination of flavones in Elsholtzia bodinieri by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop an HPLC method for the measurement of flavones in Elsholtzia bodinieri.</p><p><b>METHOD</b>Analysis was carried out on Shimadzu LC-2010A HPLC system. The separation was performed on Agilent Extend-RP C18 column (4. 6 mm x 250 mm, 5 microm), with methanol-acetonitrile-0.2% phosphoric acid as mobile phase, and the flow rate was 1.0 mL min(-1) and the detective wavelength was 280 nm.</p><p><b>RESULT</b>A good linear relationship and the mean recovery was found within the range.</p><p><b>CONCLUSION</b>This method is rapid, simple and accurate.</p>