Detection of the SRY gene by primed in situ labeling / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a primed in situ labeling (PRINS) technique which can be more effective in detection of single copy gene.</p><p><b>METHODS</b>On the basis of traditional PRINS, new reagents and procedures, such as TaqStart antibody, four primers of the sex determining region Y (SRY) gene and TSA(TM) Biotin System were included in detection of the SRY gene. Meanwhile, fluorescence in situ hybridization(FISH) to detect the SRY gene was used as control.</p><p><b>RESULTS</b>Fifty metaphases were scored. PRINS labeling showed signals for the SRY on the Y chromosome at band Yp11.3 in all metaphases. These signals were as distinct as that from results of FISH.</p><p><b>CONCLUSION</b>This improved method is ideal for rapidly localizing single copy genes and small DNA segments. And PRINS is a cost- and time-effective alternative to FISH.</p>