Changes of rat testicular germ cell apoptosis after high power microwave radiation / 中华男科学杂志
National Journal of Andrology
; (12): 407-410, 2004.
Article
em Zh
| WPRIM
| ID: wpr-308340
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of high power microwave (HPM) radiation on the testicular germ cell apoptosis.</p><p><b>METHODS</b>One hundred and twenty-five Spraque-Dawley rats were randomly divided into two groups, unexposed control group and experimental group(further divided into four subgroups: 10 mW/cm2 5 min, 10 mW/cm2 10 min, 20 mw/cm2 5 min, and 20 mW/cm2 10 min), and then the experimental group was radiated with S wave band of 10 mW/cm2, 20 mW/cm2 high power microwave for 5 or 10 min. Testicular samples were taken at 6 h, 24 h, 48 h, 72 h and 5 d after radiation and separately studied. At the end of the process, testicular germ cell apoptosis was detected by in situ terminal deoxynucleotityl transferase mediated dUTP nick end labeling (TUNEL).</p><p><b>RESULTS</b>The number of apoptotic cells of the 6 h, 24 h and 48 h experimental groups at 5 min after 10 and 20 mW/cm2 radiation was remarkably larger than that of the controls (P < 0.01), especially after 10 mW/cm2 radiation, the number of the 6 h group reached the peak (161.27 +/- 5.90) /5 convoluted seminiferous tubules. The changes in the other experimental groups had no significant difference compared with the controls (P > 0.05).</p><p><b>CONCLUSION</b>HPM can increase germ cell apoptosis of the rat testis, which is related to the time of radiation and sample acquisition. In the condition of the present test, 5 minutes of HPM radiation may significantly enhance testicular germ cell apoptosis and damage, which in turn may influence the reproductive function of the rats.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
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Efeitos da Radiação
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Espermatozoides
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Testículo
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Ratos Sprague-Dawley
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Apoptose
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Micro-Ondas
Limite:
Animals
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2004
Tipo de documento:
Article