Signal transduction pathway mediated by thrombopoietin in the inflammation model of microglia / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 340-343, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-308797
ABSTRACT
<p><b>OBJECTIVE</b>To explore the signal transduction pathway mediated by thrombopoietin (TPO) in the inflammation model of microglia induced by lipopolysaccharide (LPS).</p><p><b>METHODS</b>The inflammation model of microglia BV2 cells was prepared by LPS of 0.5 and 1.0 μg/mL stimulation. The expression of TPO and ERK mRNA in BV2 cells was detected by real time quantitative PCR. Western blot was used to evaluate the expression of TPO and ERK protein in BV2 cells. TPO and IL-6 contents in the culture supernatant fluid were measured using ELISA.</p><p><b>RESULTS</b>LPS stimulation increased significantly the mRNA and protein expression of TPO and ERK in BV2 cells, especially at the concentration of 1.0 μg/mL for 12 hrs stimulation. There was a significant positive correlation between the mRNA and protein expression of TPO and ERK.</p><p><b>CONCLUSIONS</b>Signal transduction pathway of ERK1/2 participates in the activation of TPO in inflammatory injury of BV2 cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Fisiologia
/
Trombopoetina
/
RNA Mensageiro
/
Ensaio de Imunoadsorção Enzimática
/
Transdução de Sinais
/
Microglia
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
MAP Quinases Reguladas por Sinal Extracelular
/
Genética
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2011
Tipo de documento:
Artigo
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