Culture and identification of neural stem cells from mouse embryos / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 244-247, 2011.
Article
em Zh
| WPRIM
| ID: wpr-308823
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>The purpose of this study was to culture and identify neural stem cells from mouse embryos in vitro using a modified method and provide a basis for further study of the biology of neural stem cells under hypoxia.</p><p><b>METHODS</b>The cells were isolated mechanically from the front cortex of fetal Institute of Cancer Research (ICR) mice on embryonic day 14. They were passaged by mechanical dissociation and enzymatic digestion. The neurospheres were identified by immunofluorescent staining of nestin. Cell differentiation was induced by 1% fetal bovine serum and then the cells were identified by immunohistochemistry of β-tubulin III and GFAP.</p><p><b>RESULTS</b>The cells obtained from the front cortex of fetal ICR mice had the capacity of forming neurospheres which showed nestin immunoreactive positivity. After being induced by 1% fetal bovine serum, the cells were differentiated into β-tubulin III-positive cells and GFAP-positive cells.</p><p><b>CONCLUSIONS</b>Using mechanical dissociation of primary cells and mechanical dissociation with enzymatic digestion of primary cells, the NSCs from the front cortex of mouse embryos can be obtained.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Tubulina (Proteína)
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Diferenciação Celular
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Células Cultivadas
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Química
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Biologia Celular
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Embrião de Mamíferos
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Células-Tronco Neurais
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Nestina
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Proteína Glial Fibrilar Ácida
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Proteínas de Filamentos Intermediários
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2011
Tipo de documento:
Article