Value of fluorescence quantitative PCR assay of bronchoalveolar lavage fluid samples in the diagnosis of Mycoplasma pneumoniae pneumonia in children / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 191-194, 2011.
Article
em Zh
| WPRIM
| ID: wpr-308837
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the value of fluorescence quantitative polymerase chain reaction (FQ-PCR) for detecting Mycoplasma pneumoniae (MP)-DNA in bronchoalveolar lavage fluid (BALF) in the early diagnosis of Mycoplasma pneumoniae pneumonia (MPP).</p><p><b>METHODS</b>FQ-PCR was used to detect MP-DNA in BALF obtained by fiberoptic bronchoscopy from 61 children with MPP, and the sensitivity and the specificity of FQ-PCR were compared with the traditional serological test.</p><p><b>RESULTS</b>The sensitivity and the specificity of BALF FQ-PCR for detecting MP-DNA were 94% and 100% respectively. The accuracy of BALF FQ-PCR assay for detecting MP-DNA was higher than that of the serological test at the early stage of the disease (1-7 days) (P<0.01). In the children with refractory MPP, BALF FQ-PCR assay also showed higher accuracy for detecting MP-DNA than the serological test (P<0.01). The copies of MP-DNA in children with refractory MPP were significant higher than those in children with common MPP (P<0.05). The copies of MP-DNA were positively correlated with CRP values (r=0.845, P<0.01).</p><p><b>CONCLUSIONS</b>FQ-PCR assay of BALF for detecting MP-DNA in BALF is superior to the serological test. It is a reliable method for the early diagnosis of MPP, especially refractory MPP. The copies of MP-DNA can be used as an index for evaluation of the treatment outcome of refractory MPP.</p>
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Índice:
WPRIM
Assunto principal:
Pneumonia por Mycoplasma
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DNA Bacteriano
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Líquido da Lavagem Broncoalveolar
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Reação em Cadeia da Polimerase
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Sensibilidade e Especificidade
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Dosagem de Genes
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Diagnóstico
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Fluorescência
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Métodos
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Microbiologia
Tipo de estudo:
Diagnostic_studies
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Screening_studies
Limite:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
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Newborn
Idioma:
Zh
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2011
Tipo de documento:
Article