Protective effect of calcitonin gene-related peptide against oxidative damage in MC3T3-E1 osteoblasts / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 584-588, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-309098
ABSTRACT
<p><b>OBJECTIVE</b>This study aimed to observe the protective effect of calcitonin gene-related peptide (CGRP), as well as its potential mechanism, against oxidative damage in MC3T3-E1 osteoblasts.</p><p><b>METHODS</b>1) MC3T3-E1 osteoblasts were treated with different hydrogen peroxide (H₂O₂) concentrations (10⁻¹, 10⁻², 10⁻³, 10⁻⁴, and 10⁻⁵ mol·L⁻¹) for 12, 24, 36, and 48 h to build an oxidative damage model, to determine cell proliferation activity in each group by using CCK-8 assay, and to determine the optimal modeling concentration. MC3T3-E1 osteoblasts were pretreated for 1 h with different CGRP concentrations (10⁻⁶, 10⁻⁷, 10⁻⁸, 10⁻⁹, and 10⁻¹⁰ mol·L⁻¹) followed by treatment with H₂O₂ (10⁻⁴ mol·L⁻¹). After 12, 24, 36, and 48 h, the CGRP expression and activity of osteoblasts were detected using the CCK-8 method to determine the optimal CGRP concentration that provides the best protective effect against oxidative damage. 2) Superoxide dismutase (SOD) activity, reactive oxygen species (ROS) content, and the levels of the inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 of the groups treated with CGRP, H₂O₂, CGRP+H₂O₂ were determined.</p><p><b>RESULTS</b>1) Compared with the control group, treatment with 10⁻⁴ mol·L⁻¹ H₂O₂ significantly started to inhibite the proliferation of osteoblasts (P<0.01) in a dose- and time-dependent manner. Compared with 10⁻⁴ mol·L⁻¹ H₂O₂ group, pretreatment with 10⁻⁸ mol·L⁻¹ CGRP significantly increased the proliferation of osteoblasts (P<0.01). 2) Compared with H₂O₂ group, CGRP+H₂O₂ group significantly increased the SOD activity (P<0.01), ROS content significantly decreased (P<0.01), TNF-α, IL-1β, and IL-6 secretion significantly decreased (P<0.05).</p><p><b>CONCLUSIONS</b>H₂O₂ can cause oxidative damage to MC3T3-E1 osteoblasts, whereas CGRP exerts protective effect against oxidative damage in MC3T3-E1 osteoblasts.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Calcitonina
/
Peptídeo Relacionado com Gene de Calcitonina
/
Linhagem Celular
/
Interleucina-6
/
Fator de Necrose Tumoral alfa
/
Peróxido de Hidrogênio
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2016
Tipo de documento:
Artigo
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