Preparation of hydroxyapatite/chitosan-transforming growth factor-β composite coatings on titanium surfaces and its effect on the attachment and proliferation of osteoblasts / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 229-233, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-309147
ABSTRACT
<p><b>OBJECTIVE</b>This study investigated the effects of hydroxyapatite (HA)/chitosan (CS)-transforming growth factor-β1 (TGF-β1) composite coatings on titanium surfaces, as well as on the attachment and proliferation of osteoblasts.</p><p><b>METHODS</b>HA/CS-TGF-β1 composite coatings were prepared on titanium surfaces by physical, chemical, and biological modifications. Scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform-infrared spectroscopy (FTIR), and other methods were employed to analyze the chemical composition and surface topography of the composite coatings. CCK-8 and immunofluorescence assays were used to analyze the effects of the coatings on the attachment and proliferation of osteoblasts.</p><p><b>RESULTS</b>HA/CS-TGF-β1 composite coatings were successfully prepared. Their contact angle was almost zero. These composite coatings were applied in vitro, with a drug released early and a burst release effect. The growth of osteoblasts was not inhibited on it and it had obvious promoting effect on the adhesion and early proliferation of osteoblasts.</p><p><b>CONCLUSION</b>The composite coatings significantly promote the adhesion and early proliferation of osteoblasts in vitro. This finding shows that the proposed method demonstrates a good prospective application in surface modification of titanium.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Fisiologia
/
Propriedades de Superfície
/
Titânio
/
Difração de Raios X
/
Microscopia Eletrônica de Varredura
/
Fatores de Crescimento Transformadores
/
Química
/
Estudos Prospectivos
/
Fator de Crescimento Transformador beta
Tipo de estudo:
Estudo observacional
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2016
Tipo de documento:
Artigo
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