Protective effect of maternal immunoglobulin G against NMDA-induced neurotoxicity on hippocampus neurons / 中华儿科杂志
Chinese Journal of Pediatrics
;
(12): 374-376, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-309197
ABSTRACT
<p><b>OBJECTIVE</b>To investigate possible protective effect of maternal immunoglobulin G (IgG) against N-methyl-D-aspartate-mediated neurotoxicity on primary-cultured rat hippocampal neurons and the mechanism of the effect.</p><p><b>METHODS</b>An in vitro system had been developed for the study of hippocampal neurons. Intracellular lactic dehydrogenase (LDH) release was used as a marker to measure the rates of neuronal damage. The cells were stained with Trypan blue to measure the rate of neuronal death.</p><p><b>RESULTS</b>N-methyl-D-aspartate (NMDA) at a concentration of 50 micromol/L resulted in increased release of LDH and the cell mortality (P < 0.01, respectively). Maternal IgG of different concentration (10 mg/L, 100 mg/L) inhibited NMDA-induced intracellular LDH release (P < 0.01, respectively) and cell mortality (P < 0.05, 0.01, respectively), and larger dose had stronger effect (P < 0.05).</p><p><b>CONCLUSIONS</b>Maternal IgG had protective effect on primary-cultured rat hippocampal neurons injured by NMDA and the effect was dose-dependent.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Técnicas de Cultura de Órgãos
/
Patologia
/
Farmacologia
/
Imunoglobulina G
/
Sobrevivência Celular
/
Células Cultivadas
/
N-Metilaspartato
/
Morte Celular
/
Ratos Wistar
/
Agonistas de Aminoácidos Excitatórios
Limite:
Animais
/
Gravidez
Idioma:
Chinês
Revista:
Chinese Journal of Pediatrics
Ano de publicação:
2006
Tipo de documento:
Artigo
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