Genotype of wild-type measles viruses circulating in Beijing in 2003 / 中华儿科杂志
Chinese Journal of Pediatrics
;
(12): 364-368, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-309199
ABSTRACT
<p><b>OBJECTIVE</b>To investigate genotype of wild-type measles viruses circulated in Beijing in 2003.</p><p><b>METHODS</b>Throat swabs specimens were collected from patients seen during an outbreak of measles and from clinically suspected sporadic measles patients in 2003. Vero/SLAM cell lines recommended by WHO were used to isolate measles virus. Four hundreds and fifty nucleotides of COOH-terminal of nucleoprotein (N) genes were amplified by using PR-PCR. The amplified products were sequenced and the sequences were compared with references viruses from GeneBank.</p><p><b>RESULTS</b>Eight strains of measles viruses were isolated from throat swabs of patients who came from seven districts and counties of Beijing. Sequence analysis of the 450 nucleotides of COOH-terminal of nucleoprotein (N) genes indicated that these 8 strains belonged to H1a genotype. The average genetic distances of these 8 strains to H1a genotype, Chin9322, H1b genotype, Chin9475 and H1c genotype, Chin9427, were 0.004 - 0.011, 0.026 - 0.031 and 0.015 - 0.022, respectively. The average genetic distances of these 8 strains to H1a genotype, Anhui 01 - 1/Anhui 02 - 2, were 0.000 - 0.009 (0 - 5 nucleotide variation).</p><p><b>CONCLUSIONS</b>Major genotypes of wild-type measles viruses circulated in Beijing in 2003 were H1a genotype. The genotypes H1c, H1b and H2 may have disappeared in Beijing.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Virais
/
Virologia
/
Sangue
/
Imunoglobulina M
/
RNA Viral
/
China
/
Epidemiologia
/
Surtos de Doenças
/
Classificação
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Criança
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Pediatrics
Ano de publicação:
2006
Tipo de documento:
Artigo
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