Effect of isorhmnetin on circadian rhythms of DNA synthesis and expression of c-myc gene in Eca-109 cells of human oesophageal cancer / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1227-1230, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-309915
ABSTRACT
This study was focused on the circadian rhythms of DNA synthesis and the expression of c-myc gene in untreated and treated Eca-109 cells in human oesophageal cancer with isorhmnetin. The circadian rhythms of 3H-TdR incorporation and expression of c-myc gene in untreated and treated Eca-109 cells were measured by 3H-thymidine uptake assay and flow cytometry. The data collected were analyzed by ANOVA and Cosinor method. DNA synthesis and expression of c-myc gene in untreated group varied according to circadian time with statistical significance, the distribution curves of both DNA synthesis and the expression level of c-myc were fit for cosinor changes. The circadian rhythms of DNA synthesis and circadian parameters of c-myc expression in treated Eca-109 cells changed. The circadian parameters of DNA synthesis and expression level of c-myc varied after treatment by isorhmnetin. The effects of isorhmnetin on cell proliferation and c-myc expression reached the highest level from 20 00 to 0 00. The results provide a guidance for instituting the chemotherapy and chronotherapy of human tumors, when isorhmnetin is for use as anti-cancer agent.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Quercetina
/
DNA
/
Neoplasias Esofágicas
/
Células Tumorais Cultivadas
/
Proteínas Proto-Oncogênicas c-myc
/
Ritmo Circadiano
/
Flavonóis
/
Genética
Tipo de estudo:
Guia de Prática Clínica
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2005
Tipo de documento:
Artigo
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