Analysis on the expression of the stem cells related genes in hepatocellular carcinoma cell lines / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 599-602, 2009.
Article
em Zh
| WPRIM
| ID: wpr-310038
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of Oct4, Sox2, Nanog, SMO, beta-Catenin and Wnt5b mRNA in four hepatocellular carcinoma cell lines of SMMC-7721, Bel-7402, HepG2, MHCC-97 and normal hepatocellular cell line of L02, and to compare the response of these cell lines to all-trans retinoic acid.</p><p><b>METHODS</b>RT-PCR was used to detect expression of Oct4, Sox2, Nanog, SMO, beta-Catenin and Wnt5b mRNA in four hepatocellular carcinoma cell lines and normal hepatocellular cell line. Real time-PCR was used to quantify the expression of the genes.</p><p><b>RESULTS</b>There are different levels of expression of the stem cell-related gene in hepatocellular carcinoma cell lines and control cell line (P less than 0.05). There are significant differences in HepG2 and L-02 for the response to all-trans retinoic acid (P less than 0.05).</p><p><b>CONCLUSIONS</b>The stem cell-related genes are differentially expressed in different hepatocellular carcinoma cell lines.</p>
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Índice:
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Assunto principal:
Patologia
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Farmacologia
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Células-Tronco
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Tretinoína
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RNA Mensageiro
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Transdução de Sinais
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Regulação Neoplásica da Expressão Gênica
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Carcinoma Hepatocelular
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Proteínas de Homeodomínio
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Metilação de DNA
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2009
Tipo de documento:
Article