Effect of danshensu on activation of JNK pathway in hepatic stellate cells(HSCs) induced by IL-1 beta / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 451-454, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-310062
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the inhibitory effect of danshensu on the activation of JNK signaling in rat hepatic stellate cells (HSCs) induced by IL-1beta.</p><p><b>METHODS</b>The proliferation of primary rat HSCs treated with different concentration of Danshensu was checked by MTT colorimetric assay. The expression and phosphorylation of JNK and P-JNK was detected by western blotting. Synthesis and secretion of collagen I were detected by the quantitative immunocytochemical assay and ELISA.</p><p><b>RESULTS</b>Danshensu inhibited the proliferation of HSCs in a dose-dependent manner. At the concentration of 0.0625 to 0.25 mmol/L, Danshensu significantly repressed the proliferation of HSC induced by IL-1beta (P < 0.05). Synthesis and secretion of Type I collagen was significantly decreased 24 hours after 0.25 mmol/L Danshensu treatment (P < 0.01). The phosphorylation of JNK induced by IL-1beta was significantly inhibited by Danshensu treatment (P < 0.05), however, the expression of JNK was not regulated by Danshensu.</p><p><b>CONCLUSION</b>Danshensu represses the activation and proliferation of HSCs, and inhibits the synthesis and secretion of Type I collagen, possibly via the repression of the JNK signal transduction.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Imuno-Histoquímica
/
Transdução de Sinais
/
Regulação para Baixo
/
Células Cultivadas
/
Western Blotting
/
Ratos Wistar
/
Secreções Corporais
/
Proteínas Quinases Ativadas por Mitógeno
/
Colágeno Tipo I
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2009
Tipo de documento:
Artigo
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