The effect of hepatitis B virus X protein on the c-met promoter activity in HepG2 cells / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 292-296, 2009.
Article
em Zh
| WPRIM
| ID: wpr-310107
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To confirm the effect of hepatitis B virus X (HBx) protein on the c-met promoter activity in HepG2 cells.</p><p><b>METHOD</b>The expression of c-met protein was detected by western blot in HBx-transfected HepG2 cells, the human c-met promote activity was checked by luciferase assay using five different constructs with deletion or point mutation.</p><p><b>RESULTS</b>HBx protein stimulated the expression of the c-met in HepG2 cells. The enhanced expression of c-met in HBx-transfected cells was mediated by the activation of AP-2 and SP-1 transcriptional activity at the c-met promoter region (-183bp--100bp), HBx increased the invasiveness of HepG2 cells as determined by Matrigel invasion assay.</p><p><b>CONCLUSION</b>These results suggests that HBx induces the expression of c-met through the activation of AP-2 and SP-1 activity at the promoter region; in addition, our data indicate that HBx stimulates the invasive potential of HepG2 cells.</p>
Texto completo:
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Índice:
WPRIM
Assunto principal:
Patologia
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Plasmídeos
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Fatores de Transcrição
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Transfecção
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Transativadores
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Regulação da Expressão Gênica
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Vírus da Hepatite B
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Western Blotting
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Reação em Cadeia da Polimerase
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Regiões Promotoras Genéticas
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2009
Tipo de documento:
Article