Construction of adeno-associated virus vector containing ANG-1 gene and its expression in pig mesenchymal stem cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 370-376, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-310338
ABSTRACT
<p><b>OBJECTIVE</b>To construct recombinant adeno-associated virus (rAAV) vector containing angiopoietin-1 (ANG-1) gene and to express the ANG-1 in targeting cells.</p><p><b>METHODS</b>ANG-1 cDNA was obtained from human spleen by RT-PCR and was inserted into AAV vectors to form rAAV ANG-1, the virus stocks in high titer were harvested. The rAAVANG-1 and rAAV GFP were transferred into pig mesenchymal stem cells and the expression of ANG-1 was detected by Western blot.</p><p><b>RESULTS</b>The cloned ANG-1 cDNA was 1515bp in length which was in accordance with that reported previously. Titration of rAAVANG-1 stock was 9 X 10(11)v.g/ml. The expression of ANG-1 gene was detected in transfected cells. Forty-eight hours after rAAV GFP was transfected into mesenchymal stem cells, 55% cells expressed GFP.</p><p><b>CONCLUSION</b>The constructed rAAV ANG-1 vector has successfully transfered and expressed in pig mesenchymal stem cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
Proteínas Recombinantes
/
Transfecção
/
DNA Complementar
/
Dependovirus
/
Angiopoietina-1
/
Células-Tronco Mesenquimais
/
Vetores Genéticos
/
Genética
/
Metabolismo
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2009
Tipo de documento:
Artigo
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