Fetal bovine serum enhances expression of PEDF in epidermal keratinocytes and dermal fibroblasts / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of fetal bovine serum (FBS) on expression of pigment epithelium-derived factor (PEDF) in normal epidermal keratinocytes and dermal fibroblasts.</p><p><b>METHODS</b>Keratinocytes and fibroblasts were incubated with 10% FBS. PEDF protein level in the cells was determined by immunofluorescence and Western blot.</p><p><b>RESULTS</b>PEDF was localized mostly in the cytoplasm,while some in the nuclei. The distribution of PEDF in cytoplasm was in a granular pattern. 10% FBS increased the expression of PEDF both in keratinocytes and fibroblasts,but histamine and Phorbol 12-myristate 13-acetate (PMA) did not interfere the distribution of PEDF in cells.</p><p><b>CONCLUSION</b>10% FBS can upregulate expression of PEDF in epidermal keratinocytes and dermal fibroblasts.</p>