Preparation and identification of monoclonal antibodies to staphylococcal enterotoxin I / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 265-270, 2009.
Article
em Zh
| WPRIM
| ID: wpr-310356
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To prepare and identify monoclonal antibodies against staphylococcal enterotoxin I (SEI).</p><p><b>METHODS</b>Spleen cells obtained from mice immunized with the SEI protein were fused with the myeloma cells (SP2/0). Hybridomas were screened by enzyme-linked immunosorbent assay (ELISA) and the stable monoclonal hybridomas were isolated by limiting dilution at least three times. The characters of purified monoclonal antibodies were identified by indirect ELISA and Western blotting.</p><p><b>RESULT</b>The monoclonal antibodies secreted by two hybridomas 8F7 and D8 belonged to IgG(2b) and IgG(1) subtypes. Both had high titer and specificity with no cross reaction to SEG, SEE and SEC.</p><p><b>CONCLUSION</b>The monoclonal antibodies against SEI has been successfully prepared and identified in this study.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Baço
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Staphylococcus aureus
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Imunoglobulina G
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Secreções Corporais
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Biologia Celular
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Enterotoxinas
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Alergia e Imunologia
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Hibridomas
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Camundongos Endogâmicos BALB C
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Anticorpos Monoclonais
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2009
Tipo de documento:
Article