Purification of fusion protein HSA/IL1ra and its bioactivity / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 260-264, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-310357
ABSTRACT
<p><b>OBJECTIVE</b>To purify the recombinant human serum albumin fusion protein with interleukin 1 (HAS/IL1ra) and to detect the bioactivity of the fusion protein.</p><p><b>METHODS</b>The recombinant HAS/IL1ra protein was purified by affinity chromatography and ion exchange chromatography, the bioactivity of the fusion protein was detected by IL1-induced A375 S2 cell killing.</p><p><b>RESULT</b>The purity of the fusion protein was at least 98 % as assessed by HPLC and the protective effect from the IL1-induced A375 S2 cell killing was similar to natural IL1ra.</p><p><b>CONCLUSION</b>The purified recombinant HAS/IL1ra protein in this study has a satisfactory bioactivity.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Pichia
/
Proteínas Recombinantes de Fusão
/
Albumina Sérica
/
Apoptose
/
Linhagem Celular Tumoral
/
Proteína Antagonista do Receptor de Interleucina 1
/
Genética
/
Melanoma
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2009
Tipo de documento:
Artigo
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