Effect of concanavalin A on expression of MAPEG mRNA in Balb/c mouse brain / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 255-259, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-310358
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the gene expression of MAPEG in the cortex of concanavalin A (Con A)-induced mouse immune inflammatory model and the effect of cyclosporine A (Cs A).</p><p><b>METHODS</b>Male Balb/c mouse immune inflammation model was developed by intravenous injection of Con A (20 mg/kg). Cs A (150 mg/kg) was intravenously infected prior to Con A administration. The MAPEG expressions were determined by RT-PCR.</p><p><b>RESULT</b>mGST1, mGST3, LTC(4)S, FLAP and mPGES-1 were detected by RT-PCR but not mGST2. Eight hours after Con A treatment, mGST1 level was up-regulated to 1.2 approximately 1.5 folds of control with or without Cs A treatment. mGST3ìLTC(4)S, FLAP and mPGES-1 mRNA levels were not influenced by Con A administration.</p><p><b>CONCLUSION</b>Immune mechanism may be not involved in mGST1 up-regulation in this model and Con A does not alter arachidonic acid metabolism in cortex.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Encéfalo
/
Proteínas de Transporte
/
Eicosanoides
/
Ciclosporina
/
Concanavalina A
/
Oxirredutases Intramoleculares
/
Toxicidade
/
Proteínas Ativadoras de 5-Lipoxigenase
/
Prostaglandina-E Sintases
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2009
Tipo de documento:
Artigo
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