Preparation of floxuridine loaded polycation and its antitumor activity / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To develop a new prodrug of 5-fluorouracil-polyethylenimine-beta-cyclodextrin-floxuridine (PEI-beta-CyD-Fd) and to test its antitumor activity.</p><p><b>METHODS</b>Floxuridine was conjugated to polyethylenimine-beta-cyclodextrin to form prodrug PEI-beta-CyD-Fd. The structure of synthesized PEI-beta-CyD-Fd was confirmed by (1)H-NMR, FT-IR and UV. MTT assay and cell wound healing assay were performed on human hepatic carcinoma cell line HepG2.</p><p><b>RESULT</b>The drug loading was 2 %. The MTT assay and cell wound healing assay indicated that PEI-beta-CyD-Fd significantly inhibited proliferation and migration of HepG2 cells.</p><p><b>CONCLUSION</b>The synthesized prodrug PEI-CyD-Fd has a significant antitumor activity on HepG2 cells.</p>