Peptide MC10 mediated PEI-beta-CyD as a gene delivery vector targeting to Her-2 receptor / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 7-14, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-310397
ABSTRACT
<p><b>OBJECTIVE</b>To develop a novel non-viral gene delivery vector based on polyethylenimine and beta-cyclodextrin targeting to Her-2 receptor (MC10-PEI-beta-CyD).</p><p><b>METHODS</b>The PEI-beta-CyD was synthesized by low molecular weight polyethylenimine (PEI, Mw 600) cross-linked beta-cyclodextrin (beta-CyD) via N, N-carbonyldiimidazole (CDI). The chemical linker[N-succinimidy-3-(2-pyridyldithio) propionate, SPDP] was used to bind peptide MC10 (MARAKEGGGC) to PEI-beta-CyD to form the vector MC10-PEI-beta-CyD. The (1)H-NMR was used to confirm the structure of vector. The DNA condensing ability,and the particle size of MC10-PEI-beta-CyD/DNA complexes were demonstrated by gel retardation assay and electron microscope observation (TEM). Cell viability was tested by MTT assay. The transfection efficiency was determined on cultured SKOV-3, A549 and MCF-7 cells.</p><p><b>RESULT</b>MC10 was linked onto PEI-beta-CyD successfully. The vector was able to condense DNA at N/P ratio of 5 and particle size was about (170 +/-35)nm. The vector showed low cytotoxicity and high transfection efficiency in cultured SKOV-3, A549 and MCF-7 cells.</p><p><b>CONCLUSION</b>A novel non-viral vector MC10-PEI-beta-CyD with low cytotoxicity and high transfection efficiency has been successfully synthesized.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptídeos
/
Farmacologia
/
Polietilenoimina
/
Linhagem Celular
/
Química
/
Técnicas de Transferência de Genes
/
Receptor ErbB-2
/
Marcação de Genes
/
Beta-Ciclodextrinas
/
Vetores Genéticos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2009
Tipo de documento:
Artigo
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