Higher frequency of Yq microdeletions in sperm DNA as compared to DNA isolated from blood / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 720-722, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-310459
ABSTRACT
<p><b>AIM</b>To determine if Yq microdeletion frequency and loci of deletion are similar in two tissues (blood and sperm) of different embryological origin.</p><p><b>METHODS</b>The present study included 52 infertile oligozoospermic cases. In each case, DNA was isolated from blood and sperms and polymerase chain reaction (PCR) microdeletion analysis was done from genomic DNA isolated from both the tissues. The PCR products were analyzed on a 1.8% agarose gel. PCR amplifications found to be negative were repeated at least three times to confirm the deletion of a given marker.</p><p><b>RESULTS</b>Only 1 case harbored microdeletion in blood DNA, whereas 4 cases harbored microdeletion in sperm DNA.</p><p><b>CONCLUSION</b>The frequency of Yq microdeletions is higher in germ cells as compared to blood. As the majority of infertile couples opt for assisted reproduction procreation techniques (ART), Yq microdeletion screening from germ cells is important to understand the genetic basis of infertility, to provide comprehensive counseling and most adapted therapeutics to the infertile couple.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Espermatozoides
/
Sangue
/
DNA
/
Sequências Repetitivas de Ácido Nucleico
/
Deleção de Sequência
/
Cromossomos Humanos Y
/
Genética
Limite:
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Asian Journal of Andrology
Ano de publicação:
2007
Tipo de documento:
Artigo
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