Effect of genistein on acrosome reaction and zona pellucida binding independent of protein tyrosine kinase inhibition in bull / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 650-658, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-310470
ABSTRACT
<p><b>AIM</b>To investigate if the phytoestrogen, genistein, affects essential functions of cryopreserved bovine spermatozoa.</p><p><b>METHODS</b>The effect of genistein upon motility was assessed by computer-assisted motion analysis. Hemizona assay was performed to detect the ability of spermatozoa binding to the zona pellucida. The inducibility of the acrosome reaction using progesterone and ZP3-6 peptide was analysed by fluorescein-conjugated Pisum sativum agglutinin (FITC-PSA)/Hoechst 33258 double staining. Capacitation after incubation with genistein was assessed by the chlortetracycline (CTC) assay. Immunoblots showed the pattern of protein tyrosine phosphorylation of cryopreserved bovine spermatozoa.</p><p><b>RESULTS</b>Immunodetection of tyrosine-phosphorylated proteins showed that genistein did not affect tyrosine phosphorylation in cryopreserved bovine spermatozoa. However, genistein significantly reduced the progesterone- and ZP3-6 peptide-mediated induction of the acrosome reaction and led to a dose-dependent inhibition of sperm-zona pellucida binding; while sperm motility and capacitation were not affected by this phytoestrogen, as indicated by computer-assisted sperm motion analysis and the CTC assay, respectively.</p><p><b>CONCLUSION</b>Our results suggest that in cryopreserved bovine spermatozoa, genistein affects a protein tyrosine phosphorylation-independent signal transduction pathway that is involved in sperm capacitation, the acrosome reaction and sperm-zona pellucida binding.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Capacitação Espermática
/
Zona Pelúcida
/
Proteínas Tirosina Quinases
/
Genisteína
/
Reação Acrossômica
/
Inibidores Enzimáticos
Limite:
Animais
Idioma:
Inglês
Revista:
Asian Journal of Andrology
Ano de publicação:
2007
Tipo de documento:
Artigo
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