Expression and localization of CKLFSF2 in human spermatogenesis / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 189-198, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-310521
ABSTRACT
<p><b>AIM</b>To investigate the expression and subcellular localization of chemokine-like factor superfamily 2 (CKLFSF2) in human testis and its potential role in spermatogenesis.</p><p><b>METHODS</b>A specific polyclonal antibody against CKLFSF2 was raised. The expression and cellular localization of CKLFSF2 in the seminiferous tubules was checked by immunohistochemistry method. Also, in situ hybridization was applied to localize the mRNA distribution. The EGFP-CKLFSF2 fusion protein was expressed in COS-7 cells to localize its subcellular location in vitro. In addition, the abnormal expression of CKLFSF2 in testes of patients with male infertility was assayed by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry methods.</p><p><b>RESULTS</b>Having a close correlation with spermatogenesis defects, CKLFSF2 was specifically expressed in meiotic and post-meiotic germ cells, which were localized to the endoplasmic reticulum (ER) near the Golgi apparatus.</p><p><b>CONCLUSION</b>CKLFSF2 could play important roles in the process of meiosis and spermiogenesis, and might be involved in the vesicular transport or membrane apposition events in the endoplasmic reticulum.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Espermatogênese
/
Testículo
/
Imuno-Histoquímica
/
Chlorocebus aethiops
/
Hibridização In Situ
/
Microscopia Confocal
/
Quimiocinas
/
Células COS
/
Retículo Endoplasmático
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Asian Journal of Andrology
Ano de publicação:
2007
Tipo de documento:
Artigo
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