Application of inteins in building protein affinity purification system / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1175-1184, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-310550
ABSTRACT
Intein is a part of polypeptide in the premature protein with the capability of self-splicing, which is widely applied in protein purification, protein conjuction, cyclopeptide preparation, protein labeling and biosensor. In this review, we summarized the development of intein used in protein purification, discussed intein-mediated chromatographic and non-chromatographic purification systems, and summarized the researches in manipulating intein cleavage reaction. This work is to provide clues for improvement of intein-mediated protein purification.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas
/
Cromatografia de Afinidade
/
Processamento de Proteína
/
Inteínas
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2016
Tipo de documento:
Artigo
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