Changing trends of the expression of TIMP-4 in mouse ovary during pregnant and postpartum period / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 248-252, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-310754
ABSTRACT
<p><b>AIM</b>The changes of tissue inhibitor of metalloproteinase-4 (TIMP-4) expression in mouse ovary during pregnant and postpartum period were studied to investigate the role of TIMP-4 in corpus luteum (CL).</p><p><b>METHODS</b>RT-PCR was used to deter mine the change of TIMP-4 mRNA and indirect immunofluorescence was used to observe the change of TIMP-4 protein. The expression of TIMP-4 mRNA was observed in various periods throughout the stage of pregnancy and postpartum day 1.</p><p><b>RESULTS</b>The expression of TIMP-4 was gradually enhanced from day 1 to day 8, reached a maximal expression at day 8, while decreased at day 11 and to the lowest level at postpartum day 1. Indirect immunofluorescence results further indicated that TIMP-4 protein was localized to CL and theca-intera cells in various periods throughout the pregnancy and postpartum day 1. In addition, the change pattern of TIMP-4 protein agreed with that of the TIMP-4 mRNA in pregnancy CL.</p><p><b>CONCLUSION</b>The expression of TIMP-4 in mouse ovary during pregnancy and postpartum is in spatio-temporal pattern and it may be involved in the formation and function maintain of CL during pregnancy in mice.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ovário
/
RNA Mensageiro
/
Inibidores Teciduais de Metaloproteinases
/
Período Pós-Parto
/
Genética
/
Metabolismo
/
Camundongos Endogâmicos
Limite:
Animais
/
Gravidez
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS