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Interleukin-8 inhibits clonal expansion of 3T3-L1 preadipocyte during differentiation / 中国应用生理学杂志
Chinese Journal of Applied Physiology ; (6): 243-247, 2008.
Artigo em Chinês | WPRIM | ID: wpr-310755
ABSTRACT
<p><b>AIM</b>To investigate the effect of interleukin-8 (IL-8) on the differentiation and clonal expansion of 3T3-L1 preadipocyte during the differentiation period.</p><p><b>METHODS</b>The morphological changes of 3T3-L1 cells during differentiation after the treatment of IL-8 was observed by Oil-Red O staining. Glycerol-3-phosphate dehydrogenase (GPDH) activity was measured by a spectrophotometric method. MTT method and 3H-TdR incorporation were applied to examine the changes of cell proliferation and DNA synthesis in clonal expansion of 3T3-L1 cells. Cell cycle analysis was taken by flow cytometry.</p><p><b>RESULTS</b>IL-8 could inhibit the differentiation and GDPH activity in a dose dependent manner. IL-8 decreased the cell proliferation and DNA synthesis in clonal expansion after induction. Also, the proportion of cells in G1 phase was increased and that of cells in S and G2 phase was declined after the treatment of IL-8.</p><p><b>CONCLUSION</b>IL-8 inhibits the differentiation of 3T3-L1 preadipocytes by decreasing the clonal expansion of the cells.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Ciclo Celular / Diferenciação Celular / Interleucina-8 / Adipócitos / Biologia Celular / Células 3T3-L1 / Proliferação de Células / Metabolismo Limite: Animais Idioma: Chinês Revista: Chinese Journal of Applied Physiology Ano de publicação: 2008 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Ciclo Celular / Diferenciação Celular / Interleucina-8 / Adipócitos / Biologia Celular / Células 3T3-L1 / Proliferação de Células / Metabolismo Limite: Animais Idioma: Chinês Revista: Chinese Journal of Applied Physiology Ano de publicação: 2008 Tipo de documento: Artigo