Interleukin-8 inhibits clonal expansion of 3T3-L1 preadipocyte during differentiation / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 243-247, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-310755
ABSTRACT
<p><b>AIM</b>To investigate the effect of interleukin-8 (IL-8) on the differentiation and clonal expansion of 3T3-L1 preadipocyte during the differentiation period.</p><p><b>METHODS</b>The morphological changes of 3T3-L1 cells during differentiation after the treatment of IL-8 was observed by Oil-Red O staining. Glycerol-3-phosphate dehydrogenase (GPDH) activity was measured by a spectrophotometric method. MTT method and 3H-TdR incorporation were applied to examine the changes of cell proliferation and DNA synthesis in clonal expansion of 3T3-L1 cells. Cell cycle analysis was taken by flow cytometry.</p><p><b>RESULTS</b>IL-8 could inhibit the differentiation and GDPH activity in a dose dependent manner. IL-8 decreased the cell proliferation and DNA synthesis in clonal expansion after induction. Also, the proportion of cells in G1 phase was increased and that of cells in S and G2 phase was declined after the treatment of IL-8.</p><p><b>CONCLUSION</b>IL-8 inhibits the differentiation of 3T3-L1 preadipocytes by decreasing the clonal expansion of the cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Ciclo Celular
/
Diferenciação Celular
/
Interleucina-8
/
Adipócitos
/
Biologia Celular
/
Células 3T3-L1
/
Proliferação de Células
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2008
Tipo de documento:
Artigo
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