Cdc25B overexpression reverses two-cell block in mouse embryos / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 99-103, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-310793
ABSTRACT
<p><b>AIM</b>To explore the effect of Cdc25B overexpression on the development of mouse two-cell embryos.</p><p><b>METHODS</b>The pBSK-Cdc25B was in vitro transcribed into 5'-capped mRNA for microinjection by using mMESSAGE mMACHINE kit. The Cdc25B mRNA was microinjected into mouse embryos at two-cell stage in order to observe the embryonic development and cleavage rate. Using protein kinase activity assay and Western blot to detect the MPF activity as well as the phosphorylation status of Cdc2-Tyr15 in Cdc25B overexpression group respectively.</p><p><b>RESULTS</b>The mouse embryos with Cdc25B overexpression developed to the four-cell stage 48 h after the hCG injection with the percentage of cleavage over 40% compared with the embryos in control groups which still remained at the two-cell stage. Moreover, MPF activity increased significantly after Cdc25B mRNA injection. The phosphorylation status of Cdc2-Tyr15 was coincident with MPF activity.</p><p><b>CONCLUSION</b>The results indicate that Cdc25B overexpression in early mouse two-cell embryos reverses two-cell block and promotes their development into four-cell stage by activating MPF.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Zigoto
/
RNA Mensageiro
/
Ciclo Celular
/
Divisão Celular
/
Fator Promotor de Maturação
/
Fosfatases cdc25
/
Biologia Celular
/
Desenvolvimento Embrionário
/
Embrião de Mamíferos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2008
Tipo de documento:
Artigo
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