Effect of musk soluble components on the growth, the differentiation and the transfection efficiency of rat neural stem cells (NSC) in vitro / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 25-28, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-310813
ABSTRACT
<p><b>AIM</b>To investigate the effect of musk soluble components on the growth, the differentiation and the transfection efficiency of rat neural stem cell (NSC) in vitro.</p><p><b>METHODS</b>The growth and the differentiation of rat NSC were observed when musk soluble components were added into the culture medium of NSC. Meanwhile, the pEGFP-C1, which expressed the enhanced GFP protein, was transfected into the NSC by method of electro- transfection.</p><p><b>RESULTS</b>When NSC was treated with musk soluble components, the neurites were outgrowth around NSC and attached to the plate, and the neural spheres were disassociated. The glia-like cells appeared at the concentration of 0.3 per thousand. When the concentration of musk soluble components was lower than 3 per thousand, the transformative cells could recover. Furthermore, the efficiency of transfection pEGFP into NSC was remarkably increased after the treatment with musk.</p><p><b>CONCLUSION</b>After the treatment of NSC with musk soluble components, the neural spheres were disassociated, and then attached to the plate. Musk soluble components could induce NSC differentiation into glia-like cells and improve the transfection efficiency of pEGFP-C1 in vitro.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Encéfalo
/
Ácidos Graxos Monoinsaturados
/
Materia Medica
/
Transfecção
/
Diferenciação Celular
/
Células Cultivadas
/
Química
/
Ratos Sprague-Dawley
/
Meios de Cultura
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2008
Tipo de documento:
Artigo
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