Experimental study on inhibition of restenosis by osteopontin oligopeptide antagonist after de-endothelium / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 495-499, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-310825
ABSTRACT
<p><b>AIM</b>Osteopontin 13-peptide(Gly158-Lys170), containing multi-function domains was used to inhibit the VSMC adhesion, migration. The mechanism of 13-peptide inhibiting neointima formation was investigated.</p><p><b>METHODS</b>The effect of 13-peptide on VSMC adhesion was tested by adhesion assay. The restenosis model was prepared balloon injury after administration of 13-peptide for 1 h, and then the 13-peptide was given by an intravenous drip for 7 days. The expression changes of OPN, FAK, ILK in vessel wall were detected by immunohistochemistry and Western blot.</p><p><b>RESULTS</b>The 13-peptide dose-dependently reduced adhesion of VSMC in OPN matrix, and the infiltration of macrophage in vessel wall also was reduced in the treatment group after balloon injury. The expression of OPN, FAK, ILK was down-regulated following with the inhibition of neointima thickening.</p><p><b>CONCLUSION</b>The OPN 13-peptide can inhibit inflammation and neointima formation by blocking the binding of OPN to it's receptors.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Adesão Celular
/
Células Cultivadas
/
Túnica Íntima
/
Ratos Sprague-Dawley
/
Biologia Celular
/
Modelos Animais de Doenças
/
Osteopontina
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2007
Tipo de documento:
Artigo
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